Procedure: We measured the ventillation metabolism of the tip at two opposite temperatures: (25 C) and (15 C). For distributively fish,we prepared a 250 ml jar by filling it half fully with peeing supply from the aquarium in which the fish was housed.we transfered the fish from its weighing beaker to the jar adding sufficient water to now fill the jar to about 80% of its capacity.
We position the jar in the appropiate water bath. We left the jar unstoppered, and comment the activity of the fish for 10 minutes, or until its behavior stabilized. We primed(p) a piece of sponge in the jar to garter the fish stay calm. We used a two l bottle of ice to keep the cold water pipage cold.
During this time, we became familiar with the ventilation pattern of the fish so that we were commensurate to count the movements of the gill covers (#/min.). We counted the number of gill movements in 10 seconds and multiply by 6 to obtain the number of movements per minute.
When we were reach to begin the experiment, we filled the jars, one at a time, with the stamp down water and allowed a small amount to overflow. Quickly we inserted the graduated oxygen probes and take the initial oxygen readings. We took readings on each fish at 15 minute intervals for one hour.
introductory to each reading, we observed and recorded the ventilation rate of the fish (#/min.) and recorded on the data.
Calculation:We converted oxygen dumbness readings as mg of O2 / fifty of water (ppm) to ml of O2 /liter of water by dividing each value by 1.43. we thusly divided each of the values for ml of O2/liter of water by the weight of the corresponding fish and enter it in the column ml/g.
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